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The occurrence, properties and affinities of Telfairia mosaic virus, a potyvirus prevalent in Telfairia occidentalis (Cucurbitaceae) in south western Nigeria  [1987]

Shoyinka, S.A. (International Institute of Tropical Agriculture, Ibadan (Nigeria)) Brunt, A.A. Phillips, S. Lesemann, D.-E. et al.

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Telfairia mosaic virus (TeMV) was found to occur prevalently in Telfairia occidentalis (fluted pumpkin) in farms and private gardens in and near Ibadan, south western Nigeria. It was readily transmissible in the non-persistent manner by Aphis spiraecola and by mechanical inoculation of sap to 29 of 36 species from six of eight families, it was conveniently cultured in Nicotiana benthamiana and assyed in Chenopodium quinoa or Phaseolus vulgaris. TeMV has slightly flexuous filamentous particles ca. 800 nm long which sediment as a single component of 145 S and have a buoyant density in caesium chloride of 1.32 g cm(-3). They contain c1534a. 5 per cent ss-RNA, and 95 per cent protein composed of a single polypeptide with a mol. wt. of ca. 32,000. Within infected epidermal and parenchyma cells of N. benthamiana, the particles were found scattered throughout the cytoplasm or present in small aggregates or in monolayers adjacent to the tonoplast. Infected cells also contained conspicuous cylindrical inclusions which were seen in section as "pinwheels" and "scrolls". Tube precipitin tests showed that TeMV is serologically related to, but distinct from, zucchini yellow mosaic virus (serological differentiation index 4), and distantly related to watermelon mosaic virus-2 and bean yellow mosaic virus (serological differentiation indices of 7 and 9, respectively), these relationships were confirmed by immunosorbent electron microscopy. The virus is tentatively conside
red to be a distinct potyv

From the journal

Journal of Phytopathology (Germany, F.R.)

ISSN : 0931-1785

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