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Biolistic transformation of sorghum using a rice chitinase gene [Sorghum bicolor (L.) Moench - Oryza sativa L.]
1998
Zhu, H.; Jeoung, J.M.; Liang, G.H. ; Muthukrishnan, S.; Krishnaveni, S. ; Wilde, G.
A rice chitinase gene, G11, which may have a protective role against fungal pathogens, was introduced into a sorghum inbred "Tx430". Calli derived from immature zygotic embryos were bombardaded with tungsten particles coated with a plasmid DNA containing this gene and the bar gene as a selectable marker. After transformation, six fertile transgenic plants were obtained from a total of 1,100 bombarded calli. Molecular analyses by phosphinothricin acetyltransferase (PAT) acitivity assay, Southern blotting, and western blotting confirmed the presence and expression of the selectable bar gene and the rice chitinase gene in primary transgenic plants (T0). However, gene silencing occurred in few T0 plants at certain growth stages. Progeny analysis showed that the introduced chitinase gene was inherited and segregated among T1 plants (progeny of selfed T0 plants). Western blot analysis showed a 3:1 Mendelian segregation of the rice chitinase gene in T1 progeny, suggesting that the active chitinase gene(s) was inserted in a single locus in the T0 plants
callus; cal; genetic transformation; cultivo de tejidos; tissue culture; transformation genetique; quitinasa; resistencia a la enfermedad; chitinase; transformacion genetica; methode d'amelioration genetique; culture de tissu; plasmidios; plantas transgenicas; breeding methods; genes; disease resistance; transgenic plants; sorghum bicolor; resistance aux maladies; plante transgenique; metodos de mejoramiento genetico; plasmide; gene; genetic markers; callo; plasmids; marcadores geneticos; oryza sativa; marqueur genetique
Journal of Genetics & Breeding (Italy)



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