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Chromosomal localization of rDNA in sunflower (Helianthus annuus L.) by fluorescent in situ hybridization  [1999]

Messina, R. (Udine Univ. (Italy). Dipartimento di Produzione Vegetale e Tecnologie Agrarie) Biagetti, M. (Universita della Tuscia, Viterbo (Italy). Dipartimento di Agrobiologia ed Agrochimica)

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Abstract
The rDNA probes 5.8S-18S-25S and 5S of sunflower (Helianthus annuus L., 2n=34) have been physically mapped on chromosome spreads by fluorescent in situ hybridization. The biotin-labelled 5.8S-18S-25S rDNA probe showed a strong hybridization signal on three pairs of satellited chromosome at sites corresponding to the active NORs, and on a pair of fainter, not previously reported sites. The digoxigenin-labelled 5S rDNA probe was found to hybridize on two sites, one of which lies on a satellited chromosome
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Other subjects

  • marcadores geneticos
  • gene location
  • hibridacion de adn
  • cytogenetics
  • fluorescence
  • citogenetica
  • dna
  • dna hybridization
  • IBRIDAZIONE DEL DNA
  • MARCATORI GENETICI
  • helianthus annuus
  • fluorescencia
  • genomas
  • marqueur genetique
  • localisation de gene
  • CROMOSOMI
  • genomes
  • MICROSCOPIA
  • genes
  • microscopie
  • gene
  • chromosomes
  • chromosome
  • FLUORESCENZA
  • GENOMA
  • adn
  • cytogenetique
  • localizacion de genes
  • genome
  • LOCALIZZAZIONE DEL GENE
  • CITOGENETICA
  • HELIANTHUS ANNUUS
  • DNA
  • GENI
  • hybridation d'adn
  • microscopia
  • microscopy
  • genetic markers
  • cromosomas

From the journal

Journal of Genetics & Breeding (Italy)

ISSN : 0394-9257

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Other information

Language : English

Type : Summary

In AGRIS since : 2001

Volume : 53

Issue : 3

Start Page : 259

End Page : 261

All titles :

" Chromosomal localization of rDNA in sunflower (Helianthus annuus L.) by fluorescent in situ hybridization "

" Localizzazione cromosomica di rDNA nel girasole (Helianthus annuus L.) mediante ibridazione in fluorescenza in situ "