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Antioxidant Activity and Inhibition Activity against α-Amylase and α-Glucosidase of Viola mandshurica Extracts  [apr2008]

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Lee, B.B. (Kyungnam University, Masan, Republic of Korea)
Park, S.R. (Kyungnam University, Masan, Republic of Korea)
Han, C.S. (Daehandang Herb Clinic, Sancheong, Republic of Korea)
Han, D.Y. (Daehandang Herb Clinic, Sancheong, Republic of Korea)
Park, E.J. (Kyungnam University, Masan, Republic of Korea)
Park, H.R. (Kyungnam University, Masan, Republic of Korea)
Lee, S.C. (Kyungnam University, Masan, Republic of Korea), E-mail: sclee@kyungnam.ac.kr

Abstract:
This study was performed to investigate the physiological activities of Viola mandshurica. Antioxidant activity was evaluated by measuring total phenolic contents, reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-di-2-ethyl-benzthiazoline sulphonate (ABTS) radical scavenging activity while anti-diabetic activity was measured by inhibition activities on α-amylase and α-glucosidase. V. mandshurica extracts were prepared by extracting with four different solvents (methanol, ethanol, acetone, and water). The methanol extract showed the highest total phenol content (34.49 mg/g gallic acid equivalents) among the extracts. The water extract showed the highest reducing power (0.454) at the concentration of 1,000 ㎍/mL. The acetone extract showed the most potent radical scavenging activity. DPPH and ABTS radical scavenging activity of the acetone extract at the concentration of 1,000 ㎍/mL were 21.13% and 43.53%, respectively. The inhibitory activity of acetone extracts against α-amylase and α-glucosidase showed more than 100% at the concentration of 1,000 ㎍/mL. The results indicate that V. mandshurica might have potential antioxidant and anti-diabetic activities.





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