Written Paper

Chemical components of the pouch fluid in the seahorse, Hippocampus barbouri Jordan and Richardson 1908 and their uptake in embryos  [2006]

Oconer, E.P., Mindanao State Univ. General Santos City (Philippines) Pascual, C.B. Herrera, A.A. Sakamoto, T. et al.

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The chemical composition of pouch fluid in 'pregnant' male seahorse (Hippocampus barbouri Jordan and Richardson 1908) was determined using inductively coupled plasma (ICP) and atomic absorption spectrophotometry (AAS) analyses as well as potentiometric titration. The levels of the fluid components were compared with those in the sea water. Thirteen elements (N, P, Mg, S, K, Na, Ca, Cl, Fe, Cu, Zn, Mn and B) were detected in the pouch fluid and their concentration levels differed significantly (p less than or equal to 0.05) with respect to the seawater. From the progressively decreasing levels of most macronutrients in the pouch fluid during gestation, it may be inferred that these nutrients are being utilized to provide the required elements in carrying out various physiological activities within the brooding pouch. Histochemical studies confirmed the presence of calcium deposits in the gut epithelium at late-term stages, indicating possible nutrient absorption from the pouch fluid components during embryogenesis. Data obtained can be used in the design of appropriate feeding techniques and other environmental simulation strategies for the successful breeding and culture of seahorse in captivity.

From the journal

Asian International Journal of Life Sciences

ISSN : 0117-3375