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REPROGRAMMING OF ISOLATED MICROSPORES OF PEA ONTO EMBRYOGENIC PATHWAY OF DEVELOPMENT  [2013]

Бобков, С.В.

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Pea (Pisum sativum L.) is recalcitrant species for haploid plants producing. Reprogramming of isolated microspores onto embryogenic pathway of development is a tool for solution of this problem. For this purpose stress treatments with cold (+4 grad. С) and heat (+35 grad.С) were applied to pea buds and isolated microspores, respectively. Isolated microspore cultures derived from 6 genotypes were cultivated in conditions of 23 variants of KM, NLN and MSB liquid nutrient media with different content of growth regulators, vitamins, sugars, glutamine, and casein hydrolysate. Stress treatments and composition of nutrient media supporting embryogenic pathway of microspore development were determined. Microcalli (embryoids) were produced in conditions of two nutrient media variants based on KM and MSB protocols.

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