Written Paper

About Samples in the Research of Intraspecific Genetic Diversity of Scots Pine (Pinus sylvestris L.)  [2014]

Tikhonova, I.V., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science Zatsepina, K.G. Semerikov, V.L. Semerikova, S.A., Russian Academy of Sciences. Ural Branch, Ekaterinburg (Russian Federation). Inst. of Plant and Animal Ecology et al.

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The influence of sample size on the results of the assessments of the genetic diversity of populations of Scots pine of 12 polymorphic loci allozymes and 4 chloroplast microsatellite loci (cpSSR) was investigated. According to the obtained results, the use of different genetic markers gives similar estimates of the proportion of species intrapopulation diversity. The contribution of individual variation within populations (Fst) was 98.5 % for the allozyme data and 97.9 % on cpSSR DNA markers. It was found that samples of more than 100 trees are characterized by a higher diversity of alleles. Shown was a logarithmic relationship between the number of identified chloroplast haplotypes and the volume and the number of samples in the studying area (correlation coefficient r = 0.983). An increase of up to 100 and more sample trees leads to reduction of genetic interpopulation distance Nei without significant change in the parameters characterizing the genetic structure of the species. Standard sample sizes (30-50 individuals) allow reliable estimates of the population genetic structure of the species, but not enough to study its intra-population diversity. Analysis of intrapopulation DNA genetic variation in few model populations of 300-500 trees would be useful for determining how diversity of haplotypes identified in populations depends on number of samples (therefore, on area of areal' part) and in what extent – on sample size.

From the journal

Siberian Journal of Forest Science

ISSN : 2311-1410