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Lignification in scots pine callus as reaction to cultivation conditions and nutrient medium  [2014]

Antonova, G.F., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science Zheleznichenko, T.V., Russian Academy of Sciences. Siberian Branch, Novosibirsk (Russian Federation). Central Siberian Botanical Garden Stasova, V.V., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science

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Nutrient medium composition and cultivation conditions of Scots pine (Pinus sylvestris L.) callus effect on its cells morphology, their differentiation and lignification (LC) degree was studied. Callus cells morphological characteristics, lignin content and macromolecular structure considerably vary in dependence of different factors. The increase in the duration of callus cultivation from 21 to 60 days promotes the differentiation of cells, the homogeneity of morphological cell wall structure and intensification of LC. The cultivation of callus in the darkness suppresses its growth and LC, whereas "light-darkness" condition leads to the growth of the callus and the accumulation of lignin. The callus grown in the darkness contains more low-molecular lignin and less high-molecular lignin than that cultivated in the light. The increase in sucrose concentration in nutrient medium to 5% leads to the growth of callus mass and its LC, to heightened content of high-molecular fraction in lignin and influences the composition of lignin structural units, especially under such conditions as "light-darkness" one. The effect of polyvinyl pyrrolidone (PVP) on LC depends on the development stage of callus cells, the conditions of illumination and the time of cultivation. With the cultivation in the darkness for 21 days PVP suppresses the LC, but it contributes to appearance of siringyl units in the composition of lignin. The increase in the duration of cultivation with P
VP to 60 days promotes the condensation of lignin precursors, which increases the quantity of high-molecular fraction. The ferulic acid, added in the medium, contributes to the development of the secondary wall thickening of callus cells as matrix for deposition of lignin, increases the content of low-molecular fraction and decreases the high-molecular fraction. The addition of ascorbic acid to nutrient medium favourably affects the proliferation of cells and the growth of the callus mass, but it impedes its LC.

From the journal

Siberian Journal of Forest Science

ISSN : 2311-1410