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Preliminary Results of De Novo Whole Genome Sequencing of the Siberian Larch (Larix sibirica Ledeb.) and the Siberian Stone Pine (Pinus sibirica Du Tour)  [2014]

Krutovskij, K.V., Russian Academy of Agricultural Sciences, Moscow (Russian Federation). The N.I. Vavilov Inst. of Common Genetics Oreshkova, N.V., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science Putintseva, Yu.A. Ibe, A.A. et al.

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The Illumina HiSeq2000 DNA sequencer was used for nuclear DNA sequencing in larch (Larix sibirica Ledeb.) and Siberian stone pine (Pinus sibirica Du Tour.). As a result, it was generated 2 906 977 265 high quality paired-end nucleotide sequences (reads) and 576 Gbp for Siberian larch, that corresponds to 48X coverage of the larch genome (12.03 Gbp), and 3 427 566 813 reads and 679 Gbp for Siberian stone pine, that corresponds to 29X coverage of its genome (23.6 Gbp) were obtained. These data are not enough to assemble and annotate whole genomes, but the obtained nucleotide sequences have allowed us to discover and develop effective highly polymorphic molecular genetic markers, such as microsatellite loci that are required for population genetic studies and identification of the timber origin. Sequence data can be used also to discover single nucleotide polymorphisms (SNPs). The chloroplast genomes of Larix sibirica (122561 bp) and Pinus sibirica (116704 bp) were completely assembled and annotated. First working draft assemblies of mitochondrial genome were also obtained for these two species. In addition, genome studies of high and low pathogenic forms or strains of major pathogens of the Russian boreal forests as Heterobasidion annosum s.l. and Armillaria mellea s.l. have been carried out. Genomic studies of Russian boreal forests and major phytopathogens associated with them will allow us to identify biomarkers that can be used for solving important scie
ntific and economic problems related to the conservation of forest genetic resources and breeding more resilient and fast growing trees of improved wood quality and resistance to diseases and adverse environmental factors.

From the journal

Siberian Journal of Forest Science

ISSN : 2311-1410