Written Paper

The Use of Genetic Markers of Various Types for Evaluation of Intraspecific Differentiation Level of the Siberian Spruce  [2014]

Ehkart, A.K. Kravchenko, A.N. Larionova, A.Ya., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science Semerikova, S.A. et al.

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Data on genetic diversity and differentiation degree of number of Siberian spruce (Picea obovata Ledeb.) populations from different parts of its geographic range was obtained based on variability analysis of nuclear and chloroplast DNA isoenzyme loci and microsatellite loci. Study included Siberian spruce isolated populations located on Olkhon island (Baikal lake), in Bogd-Uul natural reserve (Mongolia) and in southern part of Magadan region (Magadan State Nature Reserve) as well as populations of Siberian spruce with blue and, typical of species, green colored needles from their joint occurrence area on Baikal lake southern coast. Also analyzed were several populations of Siberian spruce from geographically-near Irkutsk and Buryatia (Vydrino, 2 и Vydrino, 1, respectively) regions. It was established that the population of Bogd-Uul significantly differentiated from other populations analyzed, including those from an isolated relic spruce population on the island of Olkhon, both isozyme and microsatellite loci nuclear and chloroplast DNA. However, the degree of differentiation of populations established using variability of the three studied markers does not always coincide. For example, the population of Olkhon island on isozyme loci reveals a fairly high level of differentiation with populations Vydrino, 2 and Krasnoyarovo of Buryatia and on microsatellite loci of chloroplast DNA is close to them, adjacent to the group populations differ slightly from eac
h other in this type of DNA markers. Significant differences in the degree of differentiation on the three various genetic markers are observed for some other comparable pairs of populations too. Thus, the results of the research showed that for obtaining a more complete and objective information about the genetic diversity and intraspecific differentiation of this widespread in Siberia species, it is advisable to use genetic markers differing in the inheritance type and the variability level.

From the journal

Siberian Journal of Forest Science

ISSN : 2311-1410