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Callus induction and plant regeneration from mature embryos of glutinous rice (Oryza sativa L.) cultivar TDK1  [2002]

Souvanh Thadavong(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy) Prapa Sripichitt(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy) Wasana Wongyai(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy) Peeranuch Jompuk(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes)

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Abstract
Present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature embryos (seeds) of glutinous rice cultivar TDK 1. It was revealed that embryos cultured on Murashige and Skoog (MS) agar medium supplemented with 2 mg/l 2,4-D and 500 mg/l L-proline under light condition produced the highest percentage of callus formation (96.91 percent). The average size of callus produced was large (6.02 mm). The calli dehydrated by placing in petridishes with covers for 7 days under light condition before transferring onto regeneration medium generated higher frequency of shoot regeneration than the calli cultured on regeneration medium without dehydration. The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 1 mg/l IAA, 4 mg/l BA and 800 mg/l casein hydrolysate which induced the highest percentage of calli forming shoots (45.00 percent) and each callus produced the largest number of shoots (average 15 shoots). The shoots were rooted at the highest rate (100 percent) when transferred onto MS agar medium supplemented with 1 mg/l NAA. The complete plantlets were thereafter transplanted to grow under greenhouse condition. They were morphologically normal and fertile.
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Other subjects

  • plant growth substances
  • oryza sativa
  • callus
  • culture media
  • growth
  • embryo culture

From the journal

Witthayasan Kasetsart (Sakha Witthayasat)

ISSN : 0075-5192

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