DPPH antioxidant assay revisited  [2009]

Sharma, Om P. Bhat, Tej K.

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Scavenging of DPPH free radical is the basis of a common antioxidant assay. A number of protocols have been followed for this assay resulting in variation in the results of different laboratories. We present a perspective of the protocols followed by different workers with incongruity in their results and recommend a standard procedure within the sensitivity range of spectrophotometry. Three common standard antioxidants viz. ascorbic acid, BHT and propyl gallate have been used in this study. The IC₅₀ values for ascorbic acid and propyl gallate were 11.8μM and 4.4μM in methanol and 11.5μM and 4.7μM in buffered methanol as reaction medium, respectively. The free radical scavenging by BHT was markedly influenced by the reaction medium. The IC₅₀ values were 60.0μM and 9.7μM when the reaction was done in methanol and buffered methanol, respectively.

Other subjects

  • standard operating procedures
  • absorbance
  • ascorbic acid
  • antioxidants
  • DPPH antioxidant assay
  • model food systems
  • butylated hydroxytoluene
  • assays
  • free radical scavengers
  • chemical analysis
  • propyl gallate
  • methanol
  • product evaluation
  • chemical composition

From the journal

Food chemistry

ISSN : 0308-8146


Other information

Language : English

Type : Journal Article

In AGRIS since : 2013

Volume : 113

Issue : 4

Start Page : 1202

End Page : 1205

Publisher : [Amsterdam]: Elsevier Science

All titles :

" DPPH antioxidant assay revisited "