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Linkage Mapping of Resistance to Reniform Nematode in Cotton following Introgression from Gossypium longicalyx (Hutch. & Lee)  [2009]

Dighe, Nilesh D. Robinson, A. Forest Bell, Alois A. Menz, Monica A. et al.

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Abstract
Reniform nematodes (Rotylenchulus reniformis Linford & Oliveira) decrease U.S. production of Upland cotton (Gossypium hirsutum L., 2n = 52, 2[AD]1) by more than US$100 million yr-1. We report here on the mapping of a gene for extreme resistance that was introgressed from the African species G. longicalyx (Hutch. & Lee, 2n = 2x = 26; 2F1). The responsible allele, designated Renlon, was localized to chromosome 11 by first screening A-subgenome simple sequence repeat (SSR) marker loci for parental polymorphism and then for association with resistance. The three most strongly coupled SSRs and a G. longicalyx gene conferring green seed fuzz, designated Fzglon, were screened against 984 resistant and susceptible individuals of multiple backcross generations. We used marker data and pedigrees to identify nonrecombinant heterozygous parents and thereby avoid bias from repeated sampling of a recombination event. We constructed linkage maps after progeny testing a small population (147) and after implementing three alternative approaches better suited to larger populations--marker-assisted genotyping analysis, applying a cut-off value as population-wide genotyping criterion, and genotype-selective sampling. The maps concordantly indicated the order to be Fzglon-Renlon-BNL3279_114-BNL1066_156-BNL836_215, with most Ren-proximal bilaterally flanking markers within 6 cM of each other. The results will clearly facilitate use of Renlon in breeding, additional mapping, gen
omics, and prospective cloning.
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Other subjects

  • pest resistance
  • Gossypium
  • Rotylenchulus reniformis
  • Gossypium longicalyx
  • cotton
  • molecular sequence data
  • introgression
  • heterozygosity
  • genetic resistance
  • interspecific hybridization
  • chromosome mapping
  • resistance genes
  • linkage (genetics)
  • Gossypium hirsutum
  • genetic markers
  • genetic polymorphism
  • wide hybridization
  • alleles
  • microsatellite repeats

From the journal

Crop science

ISSN : 0011-183X

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