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Bioassay of the chalkboard fungus Ascosphaera aggregata on larvae of the alfalfa leafcutting bee, Megachile rotundata  [1992]

Vandenberg, J.D.

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Abstract
Laboratory-reared larvae of the alfalfa leafcutting bee, Megachile rotundata, were inoculated at various ages with a range of doses of the chalkbrood fungus Ascosphaera aggregata. The calculated LD50 values (range 2.08-3.23) increased and the slopes of the dose-response curves remained the same (range 1.17-1.25) with increasing age at inoculation. For all larvae the time to death decreased with increasing dose (range 4-16 days). Larvae inoculated at 5 days of age took longer to die (average 9.5 days) than larvae inoculated at 1 to 3 days of age (average 6.7 days). A higher percentage of cadavers sporulated (95%) at intermediate doses (approximately 2000 spores/bee) than at both lower and higher doses. For larvae inoculated at 1 to 3 days of age, the weight of sporulated cadavers (an index of the number of spores) decreased as dose increased. For larvae inoculated at 5 days of age, however, dose did not affect percent sporulation or the weight of sporulated cadavers. The size of adults that survived inoculation as larvae, at any dose and age, was not different from controls.
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Other subjects

  • mycoses
  • mortality
  • larvae
  • Ascosphaera
  • Megachile rotundata
  • beneficial insects

From the journal

Journal of invertebrate pathology

ISSN : 0022-2011

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Other information

Language : English

Type : Journal Article

In AGRIS since : 2013

Volume : 60

Issue : 2

Start Page : 159

End Page : 163

All titles :

" Bioassay of the chalkboard fungus Ascosphaera aggregata on larvae of the alfalfa leafcutting bee, Megachile rotundata "