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2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vents  [2011]

Company, Rui Antúnez, Oreto Bebianno, Maria João Cajaraville, Miren P. et al.

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Abstract
Hydrothermal vent mussels Bathymodiolus azoricus are naturally exposed to toxic chemical species originated directly from vent chimneys. The amount of toxic elements varies significantly among vent sites along the Mid-Atlantic Ridge and B. azoricus must be able to adapt to changes in hydrothermal fluid composition, temperature and pressure. The aim of this work was to study changes in the proteome in the “gill-bacteria complex” of mussels B. azoricus from three hydrothermal vent sites with distinct environmental characteristics using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE). Results showed that 31 proteins had different expression profiles among vent sites and both cluster and principal component analysis confirm a clear separation of mussels between sites. This suggests the existence of specific parameters grouping individuals from the same hydrothermal site. Protein spots of the more abundant differentially expressed proteins were excised, digested with trypsin and identified by mass spectrometry. All identified proteins (actin, ubiquinone, S-adenosylhomocysteine hydrolase, cysteine peptidases, chaperonin and catalase) have been related previously with oxidative stress conditions and are known to be affected by ROS inducing stressors, including metals. Results point out to specific adaptations at the proteome level of B. azoricus depending on the level of toxicants present in their environment.
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Other subjects

  • toxicity
  • protein synthesis
  • metals
  • proteome
  • gene expression regulation
  • actin
  • ubiquinones
  • toxic substances
  • fluorescence
  • chemical speciation
  • temperature
  • gel electrophoresis
  • mass spectrometry
  • trypsin
  • mussels
  • principal component analysis
  • catalase
  • oxidative stress

From the journal

Journal of proteomics

ISSN : 1874-3919

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Other information

Language : English

Type : Journal Article

In AGRIS since : 2015

Volume : 18 v. 74

Issue : 12

Start Page : 2909

End Page : 2919

Publisher : Elsevier B.V.

All titles :

" 2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vents "