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Characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in Rana catesbeiana tadpoles  [2011]

Yamauchi, Kiyoshi Sai, Gobun

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Abstract
We investigated the interaction of 2,4,6-triiodophenol (TIP), a potent thyroid hormone disrupting chemical, with serum proteins from rainbow trout (Onchorhynchus mykiss), bullfrog (Rana catesbeiana), chicken (Gallus gallus), pig (Sus scrofa domesticus), and rat (Rattus norvegicus) using a [¹²⁵I]TIP binding assay, gel filtration chromatography, and native polyacrylamide gel electrophoresis. [¹²⁵I]TIP bound non-specifically to proteins in trout serum, specifically but weakly to proteins in bullfrog serum, and specifically and strongly to proteins in chicken, pig, and rat serum samples. Candidate TIP-binding proteins included lipoproteins (220–320kDa) in trout, albumin in bullfrog, albumin and transthyretin (TTR) in chicken and pig, and TTR in rat. TTR in the chicken, pig, and rat serum samples was responsible for the high-affinity, low-capacity binding sites for TIP (dissociation constant 2.2–3.5×10⁻¹⁰M). In contrast, a weak interaction of [¹²⁵I]TIP with tadpole serum proteins accelerated [¹²⁵I]TIP cellular uptake in vitro. Intraperitoneal injection of [¹²⁵I]TIP in tadpoles revealed that the radioactivity was predominantly accumulated in the gallbladder and the kidney. The differences in the molecular and binding properties of TIP binding proteins among vertebrates would affect in part the cellular availability, tissue distribution and clearance of TIP.
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Other subjects

  • binding sites
  • intraperitoneal injection
  • tadpoles
  • prealbumin
  • binding properties
  • Rattus norvegicus
  • trout
  • kidneys
  • gall bladder
  • Oncorhynchus mykiss
  • tissue distribution
  • chickens
  • swine
  • Lithobates catesbeianus
  • dissociation
  • gel chromatography
  • blood serum
  • thyroid hormones
  • binding proteins
  • polyacrylamide gel electrophoresis
  • rats
  • lipoproteins
  • Gallus gallus

From the journal

Comparative biochemistry and physiology

ISSN : 1532-0456

Other information

Language : English

Type : Journal Article

In AGRIS since : 2016

Volume : 153

Issue : 3

Start Page : 328

End Page : 335

Publisher : Elsevier Inc.

All titles :

" Characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in Rana catesbeiana tadpoles "