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Screening and identification of potent α-glycosidase inhibitors from Gardenia jasminoides Ellis  [2018]

Hua, D. Luo, W. Duan, J. Jin, D. et al. SAAB [Corporate Author]

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Abstract
The α-glycosidase inhibition assay is used to examine the inhibition activity of traditional Chinese medicines through extraction with methanol. The α-glycosidase inhibition activity of the isolated compounds is measured by α-glycosidase inhibition assay and compared with Acarbose. Molecular docking is implemented to study the interaction between isolated compounds and CtMGAM (maltase–glucoamylase-C) or NtMGAM (maltase–glucoamylase-N). Compared with those of Acarbose, the methanol extract of Gardenia jasminoides Ellis has a higher activity to inhibit α-glycosidase among the 74 traditional Chinese medicines. Geniposide and Gardenoside, isolated compounds from Gardenia jasminoides Ellis, have better inhibition activity (IC50 values of 0.35 and 0.46 mg/ml, respectively) than Acarbose (IC50 values of 0.75 mg/ml). The molecular docking results show that Geniposide and Gardenoside have specific binding affinities with CtMGAM and can be considered comparable to those of Acarbose. Our findings illuminate the possible anti-diabetic mechanism of Gardenia jasminoides Ellis and support its use for the treatment of diabetes.
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Other subjects

  • screening
  • glycosidases
  • chemical constituents of plants
  • acarbose
  • α-Glycosidase assay
  • Virtual screening
  • Molecular docking
  • Geniposide
  • Gardenia jasminoides
  • binding capacity
  • enzyme inhibition
  • α-Glycosidase inhibitor
  • methanol
  • computer simulation
  • Oriental traditional medicine
  • inhibitory concentration 50
  • enzyme inhibitors
  • diabetes
  • Gardenoside

From the journal

South African journal of botany

ISSN : 0254-6299