Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study  [1997]

McCleary, B.V. (Megazyme International Ireland Ltd., Bray, County Wicklow, Ireland.) Gibson, T.S. Mugford, D.C.

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An American Association of Cereal Chemists/AOAC collaborative study was conducted to evaluate the accuracy and reliability of an enzyme assay kit procedure for measurement of total starch in a range of cereal grains and products. The flour sample is incubated at 95 degrees C with thermostable alpha-amylase to catalyze the hydrolysis of starch to maltodextrins, the pH of the slurry is adjusted, and the slurry is treated with a highly purified amyloglucosidase to quantitatively hydrolyze the dextrins to glucose. Glucose is measured with glucose oxidase-peroxidase reagent. Thirty-two collaborators were sent 16 homogeneous test samples as 8 blind duplicates. These samples included chicken feed pellets, white bread, green peas, high-amylose maize starch, white wheat flour, wheat starch, oat bran, and spaghetti. All samples were analyzed by the standard procedure as detailed above; 4 samples (high-amylose maize starch and wheat starch) were also analyzed by a method that requires the samples to be cooked first in dimethyl sulfoxide (DMSO). Relative standard deviations for repeatability (RSD(r)) ranged from 2.1 to 3.9%, and relative standard deviations for reproducibility (RSD(R)) ranged from 2.9 to 5.7%. The RSD(R) value for high amylose maize starch analyzed by the standard (non-DMSO) procedure was 5.7%; the value was reduced to 2.9% when the DMSO procedure was used, and the determined starch values increased from 86.9 to 97.2%. The amyloglucosidase-alpha-amyla
se method for measurement of total starch in cereal products has been adopted first action by AOAC INTERNATIONAL

Other subjects

  • almidon
  • produit alimentaire
  • mesure
  • evaluacion
  • productos derivados de cereales
  • produit cerealier
  • experimentacion
  • evaluation
  • ensayo
  • amidon
  • medicion
  • analisis cuantitativo
  • alpha amylase
  • experimentation
  • composition globale
  • composicion aproximada
  • analyse quantitative
  • testage
  • alimentos
  • alfa amilasa

From the journal

Journal of AOAC International (USA)

ISSN : 1060-3271