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Changes of plasma membrane H+-ATPase in pathogen-resistant and pathogen-sensitive near-isogenic wheat lines in response to elicitor from stem rust [2000]
Skoczowski, A. Barna, B. Dubert, F. Niemczyk, E.
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Abstract
The aim was to investigate the effect of elicitor prepared from wheat stem rust on H+-ATPase activity in plasma membranes of near-isogenic wheat lines SrHopeRa and SrHopeSa, resistant and susceptible to stem rust race 1 pathogen, respectively. Plasmalemma fraction was purified from crude microsomal fraction by means of the phase separation in aqueous two-phase system of dextran T500 and PEG 4000 polymers. Specific H+-ATPase activity in resistant line was significantly higher than from susceptible one.