Changes of plasma membrane H+-ATPase in pathogen-resistant and pathogen-sensitive near-isogenic wheat lines in response to elicitor from stem rust
2000
Skoczowski, A.; Barna, B.; Dubert, F.; Niemczyk, E.
The aim was to investigate the effect of elicitor prepared from wheat stem rust on H+-ATPase activity in plasma membranes of near-isogenic wheat lines SrHopeRa and SrHopeSa, resistant and susceptible to stem rust race 1 pathogen, respectively. Plasmalemma fraction was purified from crude microsomal fraction by means of the phase separation in aqueous two-phase system of dextran T500 and PEG 4000 polymers. Specific H+-ATPase activity in resistant line was significantly higher than from susceptible one.
[cell membranes, genetica, triticum aestivum, resistance aux maladies, membrane cellulaire, resistencia a la enfermedad, genetique, biological differences, diferencias biologicas, genetics, disease resistance, puccinia graminis, difference biologique, membranas celulares]
[Acta Phytopathologica et Entomologica Hungarica Hungary]
2001/HU/HU2001_0.rdf
The aim was to investigate the effect of elicitor prepared from wheat stem rust on H+-ATPase activity in plasma membranes of near-isogenic wheat lines SrHopeRa and SrHopeSa, resistant and susceptible to stem rust race 1 pathogen, respectively. Plasmalemma fraction was purified from crude microsomal fraction by means of the phase separation in aqueous two-phase system of dextran T500 and PEG 4000 polymers. Specific H+-ATPase activity in resistant line was significantly higher than from susceptible one.