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Paper

Written Paper

Pilot-scale production and purification of the cellulolytic enzyme system from the white-rot fungus Phanerochaete chrysosporium  [1991]

Uzcategui, E. (University of Uppsala, Uppsala, Sweden) Raices, M. Montesino, R. Johansson, G. et al.

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The fungus Phanerochaete chrysosporium (Sporotrichum pulverulentum) was grown on a pilot scale in a 100-liter automatic/fermenter and enzyme secretion was followed by continuous HPLC monitoring on an analytical Mono Q HR 5/5 column. The fermentation process yielded a culture filtrate with cellulose-degrading enzymes in a concentration several times higher than those reported from shaken-flask cultures. A quick and efficient downstream process involving ammonium sulfate precipitation afforded high recovery of the enzymes. A new purification approach for the cellulolytic enzyme system is also presented. Three 1,4-beta-D-glucan endoglucanases (EC 3.2.1.4) and three 1,4-beta-D-glucan cellobiohydrolases (EC 3.2.1.91) are brought to physicochemical homogeneity from the crude culture filtrate in a simple two- or three-step purification procedure. Ion-exchange, hydrophobic interaction, and gel chromatography are used as the main purification methods. Two of the purified enzymes have not been reported previously in the literature

(Revue

Biotechnology and applied biochemistry (USA)

ISSN : 0885-4513

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