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COMPARISONS OF DNA ISOLATION METHODS FOR SOME FRUIT SPECIES  [2008]

Özhan ŞİMŞEK Fırat Ege KANAT Sedat SERÇE Yıldız AKA KAÇAR Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Biyoteknoloji Anabilim Dalı [Corporate Author]

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Аннотация
Virtually all molecular biology studied requires high quality DNA isolated from plants of interest. Therefore, it is important to isolate pure and high quality DNA in a short time. In this study, DNA sampled from hazelnut, avocado, persimmon, mandarin and orange were isolated using MiniPrep DNA isolation methods and its modified versions. These treatments were prepared adding 1) CTAB, 2) CTAB and PVP (CTAB+PVP), 3) CTAB and SDS (CTAB+SDS) 4) SDS to extraction buffer. The DNA samples isolated using these methods were compared for their concentrations and purity. In general, all method yielded high DNA concentrations; the differences among the fruit species and the isolation methods were found to be statistically significant. The highest DNA concentrations were recovered from hazelnut and orange while CTAB+SDS yielded significantly lower DNA concentration when compared to the other methods.
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Журнал

Derim

ISSN : 2149-2182

Other information

Язык : tur

Тип : Journal Article

In AGRIS since : 2015

Volume : 25

Issue : 1

Start Page : 59

End Page : 69

Издатель : Batı Akdeniz Tarımsal Araştırma Enstitüsü

All titles :

" COMPARISONS OF DNA ISOLATION METHODS FOR SOME FRUIT SPECIES "

" BAZI MEYVE TÜRLERİNDE DNA İZOLASYON YÖNTEMLERİNİN ETKİNLİĞİNİN KARŞILAŞTIRILMASI "