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COMPARISONS OF DNA ISOLATION METHODS FOR SOME FRUIT SPECIES  [2008]

Özhan ŞİMŞEK Fırat Ege KANAT Sedat SERÇE Yıldız AKA KAÇAR Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Biyoteknoloji Anabilim Dalı [Corporate Author]

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摘要
Virtually all molecular biology studied requires high quality DNA isolated from plants of interest. Therefore, it is important to isolate pure and high quality DNA in a short time. In this study, DNA sampled from hazelnut, avocado, persimmon, mandarin and orange were isolated using MiniPrep DNA isolation methods and its modified versions. These treatments were prepared adding 1) CTAB, 2) CTAB and PVP (CTAB+PVP), 3) CTAB and SDS (CTAB+SDS) 4) SDS to extraction buffer. The DNA samples isolated using these methods were compared for their concentrations and purity. In general, all method yielded high DNA concentrations; the differences among the fruit species and the isolation methods were found to be statistically significant. The highest DNA concentrations were recovered from hazelnut and orange while CTAB+SDS yielded significantly lower DNA concentration when compared to the other methods.
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学术期刊

Derim

ISSN : 2149-2182

其他信息

语言 : tur

类型 : Journal Article

In AGRIS since : 2015

: 25

: 1

Start Page : 59

End Page : 69

出版者 : Batı Akdeniz Tarımsal Araştırma Enstitüsü

所有题名 :

" COMPARISONS OF DNA ISOLATION METHODS FOR SOME FRUIT SPECIES "

" BAZI MEYVE TÜRLERİNDE DNA İZOLASYON YÖNTEMLERİNİN ETKİNLİĞİNİN KARŞILAŞTIRILMASI "