Phytosterols occur in relatively high concentration in the seeds of rapeseed (Brassica napus L.) and are affected by environmental and cultural factors. Nitrogen is one of the most important factors in rapeseed production. The aims of this research were to determine phytosterols levels in some new rapeseed varieties and to investigate interactions between phytosterols levels and nitrogen fertilization, in the growing season of 2004 - 05 on 19 new rapeseed varieties. The identification of phytosterols were achieved using capillary column gas-chromatography instrument. Data revealed that sitosterol (1078.8 - 1972.5 mg kg-1) was the major component, followed by campesterol (1004.7 - 1598.1 mg kg-1). Under N-fertilizer application, sitosterol content changed between 727.9 and 2104.7 mg kg-1, followed by campasterol ranged from 642.2 to 1539.0 mg kg-1. Total phytosterols content ranged between 3220 - 4147.3 mg kg-1 for N0 level; 2423.0 and 4198.4 m g kg-1 for N1. Significant interactions between genotype and nitrogen fertilization were found for the second major component campasterol and total phytosterol content. Rapeseed is rich on phytosterols. But according to high nitrogen fertilization, phytosterol content can be decreased significantly.