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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Article de revue

First Report of Sweet potato leaf curl virus in Peru  [2003]

Fuentes, S.; Salazar, L.F.;

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Leaf curling symptoms have been reported in sweet potato (Ipomoea batatas) plants infected with a geminivirus (1). Leaf curl disease appeared in Peru after the 1997 to 1998 El Niño, when the population and activity of whiteflies (Bemisia tabaci, B. argentifolii, and B. afer) increased. Approximately 6% of plants in farmers' commercial fields in San Ramón, Junín (September 2000) and Cañete, Lima (February 2001) showed typical leaf curling symptoms. Seventeen plants in total were collected from both places, and stem scions from those plants were graft-inoculated to I. setosa, which developed symptoms of leaf curling, interveinal chlorosis, and stunting. Total nucleic acid was obtained from infected sweet potato and I. setosa plants using cetyltrimethylammoniumbromide (CTAB) extraction, and primers PW285-3 (5′-CGT CGT TAG CAG TCT GCA GGC CTC CTC TAG-3′) and PW285-4 (5′ -AAC TGT AAA TAC GGA ACT GCA GTT CGA ATT-3′) for Sweet potato leaf curl virus (SPLCV), developed and provided by R. Valverde and C. Clark of Louisiana State University (2), were used to amplify SPLCV by polymerase chain reaction (PCR). Expected DNA fragments of ca. 900 bp (in all samples) and 2.4 kp (in some samples), characteristic of the subgenomic and genomic DNAs of SPLCV respectively, were obtained from symptomatic but not from symptomless (uninfected) plants. This 2.4-kb fragment was amplified in relatively small amounts compared to the 900-bp fragment. Presence of SPLCV was also confirme
d by nucleic acid spot hybridization using a full-length clone of SPLCV-US. Fourteen of 17 plants infected with SPLCV were also infected with Sweet potato chlorotic stunt virus (determined by nitrocellulose membrane enzyme-linked immunosorbent assay serological test), which is also transmitted by whiteflies. These viruses now seem to be common in farmers' fields in San Ramón and Cañete. To our knowledge, this is the first report of SPLCV in Peru.
(Revue
Plant disease
ISSN : 0191-2917

Information bibliographique

Langue:
English
Type:
Journal Article
Sur AGRIS depuis:
2015
Volume:
87
Numéro:
1
Page initiale:
98.
Editeur:
American Phytopathological Society
Tous les titres:
"First Report of Sweet potato leaf curl virus in Peru"@eng
Autre:
"Includes references"
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Information bibliographique

Langue:
English
Type:
Journal Article
Sur AGRIS depuis:
2015
Volume:
87
Numéro:
1
Page initiale:
98.
Editeur:
American Phytopathological Society
Tous les titres:
"First Report of Sweet potato leaf curl virus in Peru"@eng
Autre:
"Includes references"