The present study was aimed at determining the liver profile of blood serum of chinchillas depending on their age and health status, with special consideration of the hair coat mycosis. The experiment was based on 62 chinchillas of the standard breed aged 1 to 12 years, which were divided into two age groups: lesser 3 years and 3+ years. Blood for K3EDTA and hair cover samples were taken from all the chinchillas. In the blood serum, the activity of AspAT, ALAT, and ALP, as well as bilirubine, total protein, albumin, and cholesterol concentrations were studied. The post-slaughter livers were weighed and necropsied. It was determined that the reference activity ranges of the studied enzymes in chinchillas were: 30.72-42.36 U/l (average 34.99 U/l) for aspartate aminotransferase (AspAT), 8.29-9.64 U/l (average 8.79 U/l) for alanine aminotransferase (ALAT), and 71.52-70.83 U/l (average 71.08 U/l) for alkaline phosphatase (ALP). The activity of AspAT varied insignificantly depending on the age of chinchillas, assuming lower values in older individuals. Because of their high variability, no conclusion on the possible effect of the AspAT activity on the health status on the animals cannot be drawn. The activities of enzymes such as AspAT, ALAT, ALP, as well as the concentration of cholesterol in the blood serum cannot be compared in chinchillas and in guinea pigs because of highly different results obtained for both these species