Article de revue
The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA double-strand breaks [2012]
Jauneau , Alain(collaborateur) (, 31326 CASTANET TOLOSAN(France). 3FR3450 Plateforme Microscopie imagerie, Pôle Biotechnologie Végétale); GRIMAL , Fanny(collaborateur) (Université de Toulouse, 31024 Toulouse(France). Centre de Recherche en Cancérologie de Toulouse, UMR 1037); BOUTET , ELISA(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire); Boudsocq , François(collaborateur) (, 31062 TOULOUSE(France). Laboratoire de Biologie Moléculaire Eucaryote, UMR 5099,); et al. Université de Toulouse, 31062 TOULOUSE (France). Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, UMR 5088, [Corporate Author]
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DNA damage signaling and repair take place in a chromatin context. Consequently, chromatinmodifying enzymes, including adenosine triphosphate– dependent chromatin remodeling enzymes, play an important role in the management of DNA doublestrand breaks (DSBs). Here, we show that the p400 ATPase is required for DNA repair by homologous recombination (HR). Indeed, although p400 is not required for DNA damage signaling, DNA DSB repair is defective in the absence of p400. We demonstrate that p400 is important for HR-dependent processes, such as recruitment of Rad51 to DSB (a key component of HR), homology-directed repair, and survival after DNA damage. Strikingly, p400 and Rad51 are present in the same complex and both favor chromatin remodeling around DSBs. Altogether, our data provide a direct molecular link between Rad51 and a chromatin remodeling enzyme involved in chromatin decompaction around DNA DSBs.
