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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

Journal Article

Статья в журнале

Evidence for programmed cell death during leaf senescence in plants  [1998]

Yen, C.H.; Yang, C.H.;

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Cell death caused by senescence of leaves has been thought to be a type of programmed cell death (PCD or apoptosis) for many years, however, no studies at the nuclear level associated with PCD have been reported. In this study, leaf tissue from five different plant species, Philodendron hastatum, Epipremnum aureum, Bauhinia purpurea, Delonix regia, and Butea monosperma was used to detect the evidence of the PCD. Here, we report the detection of PCD in senescent leaf tissue. DNA ladders, resulting from the cleavage of nuclear DNA into oligonucleosomal fragments in apoptotic cells, were detected by gel electrophoresis and southern hybridization only in senescent (but not in non-senescent) leaves in all five plant species. DNA fragmentation and nuclear DNA condensation were further confirmed by using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method. Fluorescence was clearly detected in the nuclei of mesophyll cells in senescent leaves. By contrast, there was no TUNEL staining in green healthy leaves. Our results provide direct evidence to support the notion that natural senescence of the leaves is indeed an apoptotic process during plant development.
Журнал
Plant and cell physiology
ISSN : 0032-0781

Библиографическая информация

Язык:
English
Тип:
Journal Article
В АГРИСе с:
2013
Том:
39
Выпуск:
9
Стартовая страница:
922
Конечная страница:
927
Все названия:
"Evidence for programmed cell death during leaf senescence in plants"@eng
Другое:
"Includes references"
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Библиографическая информация

Язык:
English
Тип:
Journal Article
В АГРИСе с:
2013
Том:
39
Выпуск:
9
Стартовая страница:
922
Конечная страница:
927
Все названия:
"Evidence for programmed cell death during leaf senescence in plants"@eng
Другое:
"Includes references"