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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Article de revue

Developing a cell suspension system for Musa-AAA-EA cv. ‘Nakyetengu’: a critical step for genetic improvement of Matooke East African Highland bananas  [2014]

Namanya, Priver; Mutumba, G.; Magambo, S. M.; Tushemereirwe, W.;

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Embryogenic cell suspensions of triploid East African Highland bananas (Musa AAA-EA) were initiated and generated using cooking cultivar ‘Nakyetengu’ belonging to the Nakabululu clone set. Immature male flowers produced embryogenic calli consisting of embryos and friable tissue after 4 mo culture on a modified MA1 callus induction medium. Friable calli were initiated and maintained in liquid MA2 medium. A cell growth rate of 1.5–2.0 sedimented cell volume (SCV) per month was observed. Embryo development was observed at 2.18 × 10³ embryos per mL SCV. Germination of these embryos was observed at 2.8% and 6.2% for two cell suspension lines. Plant regeneration efficiency was 60–100%, all producing normal plants with a shoot and roots at weaning. In the field, somatic cell-derived plants were all normal morphology and comparable to control plants during vegetative and reproductive stages. This study is a breakthrough for recalcitrant East African Highland banana and offers a system that can provide essential raw materials for associated germplasm improvement through genetic engineering approaches.
(Revue
In vitro cellular & developmental biology
ISSN : 1054-5476

Information bibliographique

Langue:
English
Type:
Journal Article
Sur AGRIS depuis:
2015
Volume:
50
Numéro:
4
Page initiale:
442
Page finale:
450
Editeur:
Springer-Verlag
Tous les titres:
"Developing a cell suspension system for Musa-AAA-EA cv. ‘Nakyetengu’: a critical step for genetic improvement of Matooke East African Highland bananas"@eng
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Information bibliographique

Langue:
English
Type:
Journal Article
Sur AGRIS depuis:
2015
Volume:
50
Numéro:
4
Page initiale:
442
Page finale:
450
Editeur:
Springer-Verlag
Tous les titres:
"Developing a cell suspension system for Musa-AAA-EA cv. ‘Nakyetengu’: a critical step for genetic improvement of Matooke East African Highland bananas"@eng