Objective of this experiment was to study the influence of different water regimes and irrigation intervals on the growth and yield of two sesames cultivars. A split-split plot design in a completely randomized block with three replications was employed in the study. Roi-et 1 and Mahasarakham 60 cultivars, irrigation interval (3 and 7 days) and different water reqimes (Equivalent to 20, 30 and 40 mm of rainfall) were set as main plot, subplots and sub-subplots, respectively. The result showed that the total dry weight and seed yield of these cultivars were not significantly different. The Roi-et 1 gave number of capsules per plant more than those of Mahasalakham 60 but less seeds. The three-day irrigation interval gave better performance in term of total dry weight, number of capsule per plant, than the seven-day treatment. The 40 mm of rainfall treatment gave maximum dry weight, capsules per plant and seed yield.