The antioxidant and anti-diabetes effects were evaluated by the ethanol extracts from Astragalus membranaceus root classified by years using through DPPH radical scavenging activity, hydroxyl radical (ㆍOH) scavenging activity, total phenolic and flavonoid contents, reducing power activity, α-amylase and α-glucosidase inhibitory activities. IC∧50 values for DPPH radical scavenging activity of ethanol extract from 6 years old root (749.25 ㎍/mL) was higher than 1 year (1452.67 ㎍/mL) and 3 years old root (1095.61 ㎍/mL). 6 years old root showed better effects in ㆍOH scavenging activity (IC∧50: 10.58 ㎍/mL), reducing power, total phenolic contents (26.13±0.79 Tan ㎍/mg, 24.03±0.74 Cat ㎍/mg) α-amylase (33.33±0.55%) and α-glucosidase inhibitory activity (49.71±1.01%). On the other hand, total flavonoid compound contents were estimated much higher in 1 year old root (44.93±1.35 Que ㎍/mg, 70.32±2.03 Rut ㎍/mg) than others. Based on these results, It was suggested that 6 years old root of A. membranaceus has a potential candidate for functional cosmetic and medicine.