Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically signifi cant associations. Markers GWM428 and GWM11 had signifi cant effects on the phenotypic variability of heading and fl owering time during both years, which enables their practical application through MAS.