This study was carried out during the two successive seasons of 2008 and 2009, to investigate the response of Sweet Annie (Artemisia annua) to plant spacing (60 x 40, 60 x 60 or 60 x 80 cm) and fertilization using poultry manure (PM) at the rates of 10, 15 or 20 m. /fed./season, or cattle manure (CM) at the rates of 15, 25 or 35 m. /fed./season. The results showed that the widest spacing (60 x 80 cm) increased the mean fresh and dry herb yields/plant, as well as the oil percentage, oil yield/plant and artemisinin content in the leaves. The narrowest spacing (60 x 40cm) produced the highest fresh and dry herb yields/fed., and essential oil yield/fed. The application of PM at the rate of 20 m3/fed. had the best effect on the mean fresh and dry herb yields (per plant and per fed.), as well as the oil percentage, oil yield per plant and artemisinin content in leaves. The combined treatment of (60 x 80 cm) with PM fertilization at 15 m. /fed., proved to be the best treatment combined for increasing the fresh and dry herb yields per plant, as well as the oil percentage, the oil yield/plant, and the content of artemisia keton (the main oil component). On the other hand, the highest fresh and dry herb yields/fed., as well as the highest oil yield/fed., were obtained from plants grown at the narrowest spacing (60 x 40 cm), and fertilized with the highest PM rate (20 m. /fed.).