Journal Article
Antioxidant activity of phytosterols from apple seed oil [2012]
Zhao Yanwu, Engineering University of Armed Police Force, Xi’an (China), Department of Economy; Wang Xianwei, Engineering University of Armed Police Force, Xi’an (China), Department of Economy; Huang Yingzhang, Engineering University of Armed Police Force, Xi’an (China), Department of Economy;
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The research was done to study the antioxidant activity of phytosterols from apple seed oil in order to provide a theoretical basis for the screening of antioxidants and radical-scavenging mechanism of antioxidants. The research adopted DPPH•method, Fenton method and the superoxide free radical O-2• method to determine the scavenging ability of phytosterols to OH• and DPPH• by comparing with VE and BHT. Four kinds of antioxidants showed different radical scavenging activities. The ability of scavenging DPPH• was: Sterol 1(IC50=6.7 μg/mL) Sterol 2(IC50=7 μg/mL)VE (IC50=10 μg/mL) BHT (IC50=16 μg/mL). The ability of scavenging OH• was: Sterol 2(IC50=29 μg/mL)Sterol 1(IC50=30 μg/mL)BHT (IC50= 60 μg/mL)VE (IC50=85 μg/mL). The ability of scavenging O-2• was : Sterol 1(IC50=17 μg/mL) Sterol 2(IC50=19 μg/mL) BHT (IC50=21 μg/mL) VE(IC50=35 μg/mL). Phytosterols from apple seed oil had strong antioxidant properties and possessed a clear scavenging effect on DPPH•, OH• and O-2•.
