The research is aimed at discussing the effects of SOCS3 on 3T3-L1 Lipoapoptosis. With RNA extracted from mice adipose tissue as a template, SOCS3 was amplified to pMD18-T by RT-PCR and recombinant plasmid pMD18-T SOCS3 was constructed. After sequencing, SOCS3 was amplified to pEGFP-N1 with pMD18-T-SOCS3 as a template and the eukaryotic recombinant plasmid pEGFP-N1-SOCS3 was constructed, after sequencing the pEGFP-N1-SOCS3. GFP under the fluorescence microscope is expressed and the expressions of SOCS3 mRNA and protein by RT-PCR and Western blotting was used to detect the change of apoptosis. The recombinant plasmid pMD18-T-SOCS3 and pEGFP-N1-SOCS3 are qualified to be right by sequence identification in Genbank; Lipofectamine group and SOCS3 group show that GFP is expressed under the fluorescence microscopy; SOCS3 mRNA and protein in the cells detected by RT-PCR and Western blotting express significantly (P0.05). The apoptosis of SOCS3 group increased significant after Hoechst 33258 staining; Compared with the control group, SOCS3 group significantly increased apoptosis, the mRNA expression level of Bax and c-myc gene was significantly increased (P0.05), while bcl-2 and mcl-1 was significantly decreased (P0.05). SOCS3 can promote fat cell apoptosis.