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Agricultural Research, Education and Extension Organization (AREEO) was established by the Act of ‎Parliament on June 30, 1974. The main mandate of the Organization is to develop and manage all ‎research activities of agriculture and natural resources in the Ministry of Agriculture -Jahad. AREEO is ‎determined to facilitate an effective collaboration between Iranian Institutes and World’s well ‎recognized research programs in the field. It should be noted that some institutes under the umbrella ‎of AREEO had been established years earlier, such [...]

Journal Article

Artículo de revista

Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9 apple rootstock  [2003]

Miri, S. M; Vaez Livari, B, Iranian Research Organization for Science and Technology; Khalighi, A; Ghaem maghami, S. A, Iranian Research Organization for Science and Technology;

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gibberellic acid (GA3), indolebutyric acid (IBA), phloroglucinol (PG), explant orientation and culture vessels volume were studied. The basal medium was MS supplemented with 1.5 mg.1-1 BA and 1 mg.1-1 Kin. Shoot explants 1 cm in length were taken from in vitro samples. The results showed that sorbitol was more suitable as a carbohydrate for multiplication than sucrose. GA3 and PG had no effect on number of shoots, but affected length of shoots. Application of IBA had no effect on proliferation. Explant orientation has significant effect on proliferation and the best resutls were obtained with vertical orientation. Increase in vessel volume from 25 ml to 125 ml had no effect on shoots proliferation of M.9. On the basis of the results, is suggested for improving of M.9 proliferation were used from basal medium culture (MS with 1.5 mg.l-1 BA, 1 mg.l-1 Kin and 8 g.l-1 agar) containing 30 g.l-1 sorbitol, 0.1 mg.l-1 GA3, 162 mg.l-1 PG. Also, shoots put in meduim vertically.

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Información bibliográfica

Idioma:
Farsi
Tipo:
Journal Article
En AGRIS desde:
2012
Volumen:
16
Edición:
4
Página inicial:
31
Página final:
37
Todos los títulos:
" Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9 apple rootstock "
Otro:
" Summaries (Fa, En) "

Publicación

Horticulture

ISSN : 1019-9632