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University of the West Indies (Regional centre for Bahamas, Barbados, Belize, British Virgin Islands, Grenada, Guyana, Jamaica, Montserrat, Netherlands Antilles, St. Kitts and Nevis, St. Lucia, St. Vincent and the Grenadines and Trinidad and Tobago)

Journal Article

Article de revue

Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture  [1990]

Kukreja, A.K.; Mathur, A.K.; Zaim, M. (Central Institute of Medicinal and Aromatic Plants, Lucknow (India));

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A simple, defined medium has been standardized for the mass multiplication and production of virus-free plants of Pogostemon cablin (Blanco) Benth. Shoot meristems cultured on Murashige and Skoog's (1962) medium (MS) supplemented with 2.0 mg 1**-1 6-benzylaminopurine (BAP) or 0.5-2.0 mg 1**-1 6-y,y-dimethylallyaminopurine (2iP) or 0.5-2.0 mg 1**-1 zeatin (Z) and 1.0 mg 1**-1 indole-3-acetin acid (IAA) exhibited multiple shoot proliferation. Regenerated shoots readily rooted on half strength MS containing 1.0 mg 1**-1 IAA or alpha-naphthaleneacetic acid (NAA) and 1.5 % sucrose. Rooted plantlets were transferred to pots, hardened in the glasshouse and grown to maturity in the field with a high rate (80-90 %) of survival. Plant tests made for patchouli mosaic virus (PaMV) with extracts of plants developed in vitro from the fifth subculture passage onwards gave a negative reaction in diagnostic tests on the local lesion host plant, Chenopodium quinoa Willd., demonstrating the absence of the causal agent in plants regenerated from the shoot meristems.
(Revue
Tropical Agriculture (Trinidad and Tobago)
ISSN : 0041-3216

Information bibliographique

Langue:
English
Type:
Summary
Sur AGRIS depuis:
1991
Volume:
67
Numéro:
2
Page initiale:
101
Page finale:
104
Tous les titres:
"Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture"@eng
Autre:
"1 ill., 21 ref. Summary (En) *XG"
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Information bibliographique

Langue:
English
Type:
Summary
Sur AGRIS depuis:
1991
Volume:
67
Numéro:
2
Page initiale:
101
Page finale:
104
Tous les titres:
"Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture"@eng
Autre:
"1 ill., 21 ref. Summary (En) *XG"