cDNA partial sequence of HSP70 and HSP90 were isolated from bluegill Lepomis macrochirus, with highly homologous to other teleost HSP genes. RT-PCR revealed that both HSP70 and 90 mRNA of bluegill showed large distribution with minor tissue-specific variation, but regardless of sex or seasonality. This result suggests that steady expression of HSPs would be advantageous of the acclimatization or survival in the environmental changes especially for poikilothermal animals that habit in wide ranges of different water temperature.