Written Paper

Application of near infrared spectroscopy for classification of Bombyx mori L. cocoons with different fluorescence  [2008]

Atanasova, S., Thracian University, Stara Zagora (Bulgaria) Panajotov, M., Thracian University, Stara Zagora (Bulgaria) Tsenkova, R., Thracian University, Stara Zagora (Bulgaria) Agricultural Academy, Sofia (Bulgaria) [Corporate Author]

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The aim of this study was to investigate the possibility of the near infrared spectroscopy for classification of Bombyx Mori L. cocoons with different fluorescence. The investigation was carried out with 264 silk cocoons, a population of interbreeding of lines 1013х1014 with different fluorescence – violet, yellow and intermediate. The fluorescence of cocoons was determined by a UV lamp. The near infrared diffuse reflectance spectra of cocoons in the spectral region 400-2500 nm were obtained by NIRSystem 6500 spectrometer. The SIMCA method was used for classification of the samples according to their fluorescence. Two-thirds of the analyzed samples were used as a calibration data set, and the remaining ones as a validation set. A very high accuracy – from 96.5 to 100% correct classifications, was achieved using the spectral region from 600 to 1100 nm and the first derivative data transformation. The spectral differences between the cocoons with different fluorescence were obtained. The spectral data at 784, 820, 898 and 1014 nm were found to be most significant for distinguishing between the classes.

From the journal

Journal of Animal Science

ISSN : 0514-7441