Written Paper

Calcium-calmodulin and the inducement of heat shock proteins in wheat seedling  [2000]

Fan Zhihe Zhou Rengang Li Xiaozhi (Hebei Provincial Academy of Agricultural and Forestry Sciences, Shijiazhuang (China). Inst. of Agro-Physics, Physiology and Biochemistry)

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The calmodulin (CaM) level in wheat seedlings was found to increase remarkably during heat shock at 34 degrees centigrade in the presence of Ca2+, reaching its maximum after 90 min of heat shock. The Ca2+ chelator EGTA prevented CaM accumulation at 34 degrees centigrade (Fig. 1). Pretreatment of wheat seeds with EGTA or CaM antagonist CPZ, TFP decreased the synthesis of heat shock proteins in seedlings formed from them kept at 34 degrees centigrade (Figs. 2, 3). Wheat seedlings subjected to heat shock at 34 degrees centigrade or 37 degrees centigrade developed acquired thermotolerance, but the pretreatment of seeds with EGTA, the Ca2+ channel blocker Verapamil, the CaM antagonist TFP or CPZ eliminated the heat-shock-induced thermotolerance (Figs. 4, 5). These results suggest that Ca2+-calmodulin may be involved in the synthesis of heat shock proteins and acquisition of the heat-shock-induced thermotolerance

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ZhIwu ShEngli Xuebao (China)

ISSN : 0257-4829