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The Chinese Academy of Agricultural Sciences (CAAS) is a national, integrative agricultural scientific research organization with responsibility for carrying out both basic and applied research, as well as research into new technologies impacting agriculture. CAAS is dedicated to overcoming a broad range of challenges impacting agricultural development and support of the local rural economy. Importantly, CAAS promotes sustainable agriculture within and outside China, extending its reach through technology exchange and cooperative research agree [...]

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Journal Article

Journal Article

Construction of cDNA library for tender roots of tea plant ?Camellia sinensis ?L.? O. Kuntze? and analysis of its expressed sequence tags  [2009]

Shi Chengying, Anhui Agricultural University, Hefei(China),Key Laboratory of Tea Biochemistry and Biotechnology; Wan Xiaochun, Anhui Agricultural University, Hefei(China),Key Laboratory of Tea Biochemistry and Biotechnology; Jiang Changjun, Anhui Agricultural University, Hefei(China),Key Laboratory of Tea Biochemistry and Biotechnology;

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Based on SMART technology, we constructed a cDNA library from the tissue of tender roots of tea plant ? Camellia sinensis?. The results showed? without amplication of the library, the cloning efficency was 5 ?10. , the recombinant rate was 92% , and the average size of inserted cDNA fragment was over 1 kb. It suggested that one high quality tender roots cDNA library of tea plant was successfully constructed. Then a set of clones were randomly selected and single-pass sequenced from their 5? end. As a result, 2 651 clones were sequenced and 2 600 valid sequences were generated with average size of 703 bp after vector trimming and discarding the sequences less than 100 bp, in which 404 contigs and 610 singlets were obtained after initial assembly with Phrap program. Blastx was performed against a nonredundant protein sequence database in NCBI and filtered at e-value of 1 e-10, 734 known functional genes, 102 putative functional genes and 178 unknown mRNA were achieved. This study makes a foundation for isolation of functional genes especially those related with metabolism and control in the root of tea plant.
From the journal
Journal of Nanjing Agricultural University
ISSN : 1000-2030

Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2011
Volume:
32
Issue:
1
Start Page:
126
End Page:
130
All titles:
"Construction of cDNA library for tender roots of tea plant ?Camellia sinensis ?L.? O. Kuntze? and analysis of its expressed sequence tags"@eng
"????cDNA??????EST??"@chi
Other:
"Resume(Zh,En)"
"16 ref."
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Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2011
Volume:
32
Issue:
1
Start Page:
126
End Page:
130
All titles:
"Construction of cDNA library for tender roots of tea plant ?Camellia sinensis ?L.? O. Kuntze? and analysis of its expressed sequence tags"@eng
"????cDNA??????EST??"@chi
Other:
"Resume(Zh,En)"
"16 ref."