This thesis developed a solid phase extraction-high performance liquid chromatography（SPE-HPLC） method for the determination of Kaempferol in rabbit plasma.In the method,β-Glucuronidase and sulfatase were used to hydrolyze plasma samples before analysis,and Kaempferol in plasma was extracted with a octadecylsilyl C18 SPE column.The mobile phase was methanol.0.4% phosphoric acid（55∶45） with the flow rate of 1 mL・min. 1,and the detection wavelength was set at 360 nm.The results showed that the optimum enzymatic hydrolysis conditions were β-Glucuronidase concentration 400 U・mL. 1,and sulfatase concentration 20 U・mL. 1 for 2 h respectively,the calibration curves for Kaempferol in plasma were liner between 0.019-0.608 g・mL. 1;the assay recovery was 80.23% to 84.27%;the intra-day and inter-day precisions（RSD） were all less than 10.17%.It could be concluded this method had good sensitivity and precision for the determination of Kaempferol in plasma.It was shown to be for pharmacokinetics studies of Kaempferol.[著者文摘]