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The Chinese Academy of Agricultural Sciences (CAAS) is a national, integrative agricultural scientific research organization with responsibility for carrying out both basic and applied research, as well as research into new technologies impacting agriculture. CAAS is dedicated to overcoming a broad range of challenges impacting agricultural development and support of the local rural economy. Importantly, CAAS promotes sustainable agriculture within and outside China, extending its reach through technology exchange and cooperative research agree [...]

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Journal Article

Journal Article

Optimizing Cultivation of Mature Adipocytes and Obtaining Preadipocytes by Mature Adipocyte Dedifferentiation in Pigs(Sus scrofa)  [2012]

Wang Huanhuan, Nanjing Agricultural University?Nanjing(China)?College of Animal Science and Technology; Sun Wenxing, Nanjing Agricultural University?Nanjing(China)?College of Animal Science and Technology; Xu Chunying, Nanjing Agricultural University?Nanjing(China)?College of Animal Science and Technology;

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The mature adipocyte dedifferentiation is able to provide homogeneous preadipocyees for researching adpocyte differentiation. In this study, mature adipocytes were isolated and cultured from adipose tissue, and then dedifferentiated to achieve preadipocytes. The subcutaneous adipose tissue from 3-day-old piglets(Sus scrofa) were digested by collagenase type ?, followed by centrifugation at different centrifugal force and then cultured by "ceiling culture" method. Morphological changes of adipocytes were observed under microscope, and the degree of adipogenesis and differentiation was assessed via oil red O staining. By inducing, adipose-derived progeny cells were redifferentiated into lipid-laden cells with accumulation of lipids. In terms of expression level, peroxisome proliferator-activated receptor-? (PPAR?) and fatty acid binding protein 4 (FABP4) were detected by Real-time PCR. As we expected, the adipogenic markers, PPAR? and FABP4, increased along with the redifferentiation process. Particularly, a 2.8-fold increase of PPAR? expression, and a 62-fold of FABP4 were shown in the late phase of the redifferentiation process, demonstrating the significantly higher expression levels comparing with the unredifferentiated cells at 0 d(P0.05). Showing that the predipocytes obtained by dedifferentiation might effectively differentiate to mature adipocytes with adipogenic induction agent. In addition, our study optimized the mature adipocytes culture system a
s well. Using this system, mature adipocytes can revert into proliferative-competent progeny cells by inducing redifferentiate again into mature adipocytes, which contributes an in vitro model for adipocytes investigation.
From the journal
Journal of Agricultural Biotechnology
ISSN : 1674-7968

Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2013
Volume:
20
Issue:
8
Start Page:
915
End Page:
921
All titles:
"Optimizing Cultivation of Mature Adipocytes and Obtaining Preadipocytes by Mature Adipocyte Dedifferentiation in Pigs(Sus scrofa)"@eng
"?????????????????"@chi
Other:
"Resume(Zh,En)"
"18 ref."
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Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2013
Volume:
20
Issue:
8
Start Page:
915
End Page:
921
All titles:
"Optimizing Cultivation of Mature Adipocytes and Obtaining Preadipocytes by Mature Adipocyte Dedifferentiation in Pigs(Sus scrofa)"@eng
"?????????????????"@chi
Other:
"Resume(Zh,En)"
"18 ref."