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The Chinese Academy of Agricultural Sciences (CAAS) is a national, integrative agricultural scientific research organization with responsibility for carrying out both basic and applied research, as well as research into new technologies impacting agriculture. CAAS is dedicated to overcoming a broad range of challenges impacting agricultural development and support of the local rural economy. Importantly, CAAS promotes sustainable agriculture within and outside China, extending its reach through technology exchange and cooperative research agree [...]

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Journal Article

Journal Article

Construction of Myostatin gene targeting vector of mouse  [2012]

Li Sanhua, ZunYi Medical College, ZunYi ?China?, Research Center for Medicine and Biology; Ling Xin, ZunYi Medical College, ZunYi ?China?, Research Center for Medicine and Biology; He Zhiquan, ZunYi Medical College, ZunYi ?China?,Department of Histology and Embryology;

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[Objective] This study aimed to construct Myostatin (MSTN) gene targeting vector of mouse. [Method] Total RNA was extracted from hindlimb muscle tissues of mouse to synthesis cDNA as the template to clone the coding region of MSTN. The CDS of MSTN gene including 3 kb 5? homologous arm and 1.4 kb 3? homologous arm were inserted into vector pBluescript_SK+ to construct the targeting vector pLoxP-5N3T-M. The neo and HSV-tk gene were cloned into vector pBluescript_SK+ as positive and negative selective gene. [Result] Restriction enzyme digestion and sequencing results show that mouse MSTN gene is cloned into the targeting vector pLoxP-5N3T-M. [Conclusion] The mouse MSTN gene targeting vector pLoxP-5N3T-M was successfully constructed.
From the journal
Agricultural Science and Technology
ISSN : 1009-4229

Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2014
Volume:
13
Issue:
6
Start Page:
1164
End Page:
1166
All titles:
"Construction of Myostatin gene targeting vector of mouse"@eng
"????????????????"@chi
Other:
"Resume (Zh,En)"
"6 ref."
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Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2014
Volume:
13
Issue:
6
Start Page:
1164
End Page:
1166
All titles:
"Construction of Myostatin gene targeting vector of mouse"@eng
"????????????????"@chi
Other:
"Resume (Zh,En)"
"6 ref."