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The Chinese Academy of Agricultural Sciences (CAAS) is a national, integrative agricultural scientific research organization with responsibility for carrying out both basic and applied research, as well as research into new technologies impacting agriculture. CAAS is dedicated to overcoming a broad range of challenges impacting agricultural development and support of the local rural economy. Importantly, CAAS promotes sustainable agriculture within and outside China, extending its reach through technology exchange and cooperative research agree [...]

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Journal Article

Journal Article

Cloning and sequence analyses of myostatin gene in Squaliobarbus crriculus  [2013]

Shao Fang, Changshu Institute of Technology,Changshu(China),College of Biological Science and Food Technology; Yu Jianfeng, Changshu Institute of Technology,Changshu(China),College of Biological Science and Food Technology; Zhang Yanping, Changshu Institute of Technology,Changshu(China),College of Biological Science and Food Technology;

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Myostatin is a negative regulator of skeletal muscle growth and has a potential application in aquaculture.In order to clone full-length myostatin gene and characterize its tissue expression profile in Squaliobarbus crriculus,RT-PCR and rapid-amplification cDNA ends?RACE?were used to acquire a full-length of 2 214 bp myostatin cDNA,which included a 1 128 bp long complete ORF encoding a 375 amino acid peptide.myostatin gene in S.crriculus consisted of two typical TGF-? protein domains?TGF-? propeptide domain and TGF-? activity domain.Multiple sequence comparison showed that the S.crriculus myostatin had a high homology with Cyprinus carpio at 96.99%.A homology with other fish at 76%-81%,and a low homology with mammals at 63%.S.crriculus myostatin protein amino acid sequence had a very high homology with other species,especially in the C terminal biologically active region.In the eight examined tissues,the myostatin gene was highly expressed in muscle and brain,followed by in heart,weakly expressed in gill,spleen and intestine,and traced in kidney,and there was no obvious expression in liver.These results suggested that the fish myostatin gene might not only play roles in muscle development but also contribute to other biological functions.
From the journal
Journal of Nanjing Agricultural University
ISSN : 1000-2030

Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2014
Volume:
36
Issue:
1
Start Page:
108
End Page:
114
All titles:
"Cloning and sequence analyses of myostatin gene in Squaliobarbus crriculus"@eng
"???myostatin????????????"@chi
Other:
"Resume(Zh,En)"
"22 ref."
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Bibliographic information

Language:
Chinese
Type:
Journal Article
In AGRIS since:
2014
Volume:
36
Issue:
1
Start Page:
108
End Page:
114
All titles:
"Cloning and sequence analyses of myostatin gene in Squaliobarbus crriculus"@eng
"???myostatin????????????"@chi
Other:
"Resume(Zh,En)"
"22 ref."