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El Instituto de Investigaciones de Sanidad Vegetal (INISAV), con más de 20 años aportando resultados a la agricultura cubana, es el soporte científico-técnico y metodológico del Servicio Estatal de Sanidad Vegetal, principalmente en el diagnóstico fitosanitario, la introducción y producción de medios biológicos, la implantación de Programas de Manejo Integrado de Plagas y el monitoreo de la calidad y los residuos de plaguicidas. Son beneficiados con sus resultados los agricultores asociados a las diferentes formas y tipos de producción agraria. [...]

Journal Article

Journal article

Phytophthora palmivora damaging avocado trees: first report in Cuba  [2209]

Machado, M., Instituto de Investigaciones de Fruticultura Tropical, Ciudad de La Habana (Cuba); López, M.O., Instituto de Investigaciones de Sanidad Vegetal, Ciudad de La Habana (Cuba); Collazo, C., Instituto de Investigaciones de Fruticultura Tropical, Ciudad de La Habana (Cuba); Peña, M., Instituto de Investigaciones de Fruticultura Tropical, Ciudad de La Habana (Cuba); et al.

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The oomycete genus Phytophthora cause some of the most devastating plant diseases in the world. Root rot caused by P. cinnamomi is one of the most important diseases affecting avocado trees. However, other species such as P. citricola and P. heveae have also been associated with diseases of this crop. In Cuba, despite root rot is considered a very common disease, there are just few reports about the presence of its causal agent. In the present study a morphological, physiological and molecular characterization of six fungal samples isolated from the rhizosphere of symptomatic avocado trees collected at Jagüey Grande region as well as from an affected fruit sampled in Alquizar, was carried out. Morphological and physiological features of Cuban isolates agreed with those described for P. palmívora (Butler). Typical molecular fragments of about 900 pb, obtained using ITS5/ITS4 primers confirmed their classification into the Phytophthora genus. PCR amplification primed with Lpv3f/ Lpv3r specific to P. cinnamomi, P. palmivora, P.capsici and P. hibernalis yielded a band corresponding to the expected size. Sequence analysis of the amplified product from the specific PCR reaction of one isolate showed a 99% of similarity with P. palmivora. To actual knowledge this is the first report of this species affecting avocado plants in Cuba. Its role as a pathogen is still discussed.

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ISSN : 1562-3009