AGRIS

Data provider:

Icon data provider

Unique in the Czech Republic, the third greatest agricultural library all over the world concerning its significance and the extent of the collection, information sources such as 1.1 mil. of publications, created databases, and the extensiveness of provided services for the area of agricultural and food sector and the related fields. Access point to the complex series of specialised and branch information, publications and databases available online via internet. Deposit FAO library for the Czech Republic. National centre of the Internationa [...]

Active (Data provider submitted metadata in the last calendar year)
Journal Article

Journal Article

Simultaneous analysis of bovine growth hormone and prolactin alleles by multiplex PCR and RFLP  [1998]

Chrenek, P.; Vasicek, D.; Bauerova, M.; Bulla, J. (Vyskumny Ustav Zivocisnej Vyroby, Nitra (Slovak Republic));

Access the full text

  • NOT AVAILABLE
A simplified method of simultaneous genotyping of bovine growth hormone (bGH) and prolactin (bPRL) has been developed. The method is based on simultaneous amplification of fragments of the two genes by multiplex PCR, and subsequent digestion of the products by two restriction enzymes (Alu I and Rsa I). Digestion with Alu I distinguished growth hormone L and V alleles and did not cut within the prolactin fragment, while digestion with Rsa I allowed the differentiation between the A and B alleles of bovine prolactin. All combinations of the known alleles could be detected by electrophoretic separation on the same agarose gel. Frequency of the allele L of the bovine growth hormone gene in Slovak Pied, Slovak Pinzgauer and Holstein was 0.55, 0.55 and 0.72, respectively. Frequency of the allele A of the bovine prolactin gene in the same breeds was 0.87, 0.68 and 0.95, respectively.
From the journal
Czech Journal of Animal Science - UZPI (Czech Republic)
ISSN : 0044-4847

Bibliographic information

Language:
English
Type:
Summary
In AGRIS since:
1998
Volume:
43
Issue:
2
Start Page:
53
End Page:
55
All titles:
"Simultaneous analysis of bovine growth hormone and prolactin alleles by multiplex PCR and RFLP"@eng
Other:
"Summaries (En, Sk)"
"1 graph; 19 ref."
Loading...

Bibliographic information

Language:
English
Type:
Summary
In AGRIS since:
1998
Volume:
43
Issue:
2
Start Page:
53
End Page:
55
All titles:
"Simultaneous analysis of bovine growth hormone and prolactin alleles by multiplex PCR and RFLP"@eng
Other:
"Summaries (En, Sk)"
"1 graph; 19 ref."