AGRIS

Data provider:

Icon data provider

Estonian University of Life Sciences Library is a research and public library that ensures the availability of study and scientific literature in the fields related to the bioeconomy, sustainable use of natural resources and the development of rural life and rural economy.

Interrupted (Data provider is temporarily inactive and did not submit metadata for at least last calendar year)
Paper

Written Paper

Vitrification in tissue cultures  [1999]

Vardja, R. (Estonian Agricultural Univ., Tartu (Estonia). Inst. of Experimental Biology);

Access the full text

  • NOT AVAILABLE
Vitrification is a physiological disturbance affecting tissue culture-based propagation of several plant species. Vitrified plants have a glassy appearance, their stems and leaves are enlarged, thick, translucent and brittle. The morphological malformations are associated with chlorophyll deficiency, poor lignification and excessive hydratation of tissues. The reasons for the occurrence of vitrification in tissue-culture plants is not clear. Several hypotheses have been offered to explain the cause of this physiological defect including high levels of cytokinis, low concentration of gelling agents liquid medium and excess mineral elements. Various measures were found to reduce vitrification: increased carbohydrate levels in the medium, changed light intensity, modified concentration of agar, reduced humidity within the culture container. Recently it was shown that various bacterial species and polisaccharides extracted from them can also reduce hyperhydricity. Vitrification is a complex phenomen depending on several chemical and physical factors acting together. The availability of water appears to be the central causal link

Bibliographic information

Type:
Summary
In AGRIS since:
2002
Start Page:
104
End Page:
119
Publisher:
Ltd. Rebellis
All titles:
"Vitrification in tissue cultures"@eng
Other:
"Summary (En)"
"74 ref."
"Plant tissue cultures: research and applications, Estonian Agricultural University, Tartu (Estonia). Inst. of Experimental Biology.- Harku (Estonia): Ltd. Rebellis, 1999.- ISBN 9985-882-40-7. 229 p."
Loading...

Bibliographic information

Type:
Summary
In AGRIS since:
2002
Start Page:
104
End Page:
119
Publisher:
Ltd. Rebellis
All titles:
"Vitrification in tissue cultures"@eng
Other:
"Summary (En)"
"74 ref."
"Plant tissue cultures: research and applications, Estonian Agricultural University, Tartu (Estonia). Inst. of Experimental Biology.- Harku (Estonia): Ltd. Rebellis, 1999.- ISBN 9985-882-40-7. 229 p."