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The Ethiopian Agricultural Research is one of the oldest and largest agricultural research system in Africa. Ethiopian Agricultural Research System (EARS) has evolved through several stages since its first initiation during the late 1940s, following the establishment of agricultural and technical schools at Ambo and Jimma. In 1955, a full-fledged agricultural experiment station was established at Debre Zeit (now named Debre Zeit Agricultural Research Center) under the then Imperial College of Agricultural and mechanical Arts (now called Haramay [...]

Thesis

Thesis

EFFECT OF N-PHENYL-N__- (2-CHLORO-4-PYRIDYL) UREA ON GROWTH AND ARTEMISININ CONTENT IN THE HAIRY ROOT AND TRANSPLANTED ARTEMISIA ANNUA L.  [2010]

Elias Gebremariam(Researcher); Mahidol University [Corporate Author]

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Artemisinin, a sesquiterpene lactone endoperoxide compound derived from Artemisia annua L., is highly effective against multi-drug resistant Plasmodium spp. Enhancement of artemisinin content in plant parts produced from different culturing systems was investigated by using plant bioregulator in order to provide other alternative sources of artemisinin production besides the field grown plants. Three kinds of plant bioregulator, which identified as synthetic cytokinin as N-phenyl-N′-(2 -chloro-4-pyridyl) urea (CPPU), gibberellic acid (GA3) and the anti-gibberellin compound, paclobutrazol (PBZ) at different concentrations were applied to the hairy root culture of A. annua at the late exponential phase or 10-14 days after subculture. Artemisinin content was analyzed using high performance liquid chromatography (HPLC) after 0, 24, 48 and 72 h. The artemisinin content that could not normally be detected in the hairy root culture of A. annua was increased in responses to CPPU and GA3 but not PBZ. Highest artemisinin content was obtained from the hairy root culture treated with 20.2 µM of CPPU after 48 h, which was 4-fold higher than the untreated culture. GA3 treatment at 14.5 µM could enhance artemisinin production after 72 h, however the amount of artemisinin contents in CPPU treated the hairy root culture was higher than those treated with GA3. The effect of CPPU was further investigated using pot plants after 30 days of transplantation from in vitro culture
s. Eight different mutant lines of transplanted A. annua were treated with 20.2 µM CPPU, artemisinin content and pigment compositions were then evaluated by HPLC and UV-spectrophotometer, respectively. Application of CPPU to transplanted A. annua affected to alter contents of artemisinin, total chlorophyll, total carotenoids, and anthocyanin, which displayed differently among plant lines. The present study showed the potential use of CPPU in promoting artemisinin accumulation in both the hairy root system and transplanted pots of A. annua. It was also suggested that using effective stimulators with appropriate culturing system could enhance not only production of the target compound but also other useful compounds.