An efficient plant regeneration protocol has been developed from nodal explants of Ocimum basilicum L., medicinally important herbaceous plant species belonging to the family Lamiaceae. Axillary shoot bud proliferation was initiated from nodal explants cultured on Murashige and Skoog (MS) medium supplemented with various concentration of benzyl adenine (BA) (0.25_2.0 mg 1−1) and kinetin (KN) (0.25_2.0 mg 1−1). Maximum numbers of shoots (6.2±0.1) with average length (3.7±0.0) were induced on medium containing 0.5 mg 1−1 BA. Shoots culture was established by repeated subculturing the original nodal explant on the same medium. Rooting of shoots was achieved on half strength MS medium supplemented with 1.0 mg 1−1 NAA. Well-developed complete plantlets were transferred to plastic pots containing a mixture of (1:1) soil and vermiculite showed 90% survival rate.