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Journal Article

Journal article

Phenotypic Characteristics of Pasteurella Multocida Isolated From Commercial Chickens Affected By Fowl Cholera in Jos, Nigeria  [2015]

Dashe Yakubu; Raji Moshood; Abdu Paul; Oladele Blessing; et al.

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This study was conducted to isolate and study the phenotypic characteristics Pasteurellamultocida organism recovered from commercial chicken with cases of fowl cholera in Jos,Nigeria. A total of 2000 samples consisting of bone marrow, heart, liver, lung and spleen (400each) were collected from 400 clinically sick chickens for the isolation of P. multocida. Swabfrom each sample was cultured on 7% defibrinated sheep blood, MacConkey and casein sucroseyeast agar. Presumptive colonies of P. multocida were subjected to biochemical characterizationand Microbact test. Disk diffusion method was employed to test for the sensitivity of the 12 P.multocida isolates confirmed by biochemical and Microbact test. The 12 isolates of P. multocidawere tested for their sensitivity against 15 different antibiotics. Drug sensitivity test revealed thatciprofloxacin, streptomycin and gentamicin were (100%) highly effective against the 12 P.multocida isolates. High resistance of P. multocida was recorded for ampicillin 91.7%,amoxicillin/clavulanic acid 83.3% and trimethoprim/sulfamethoxazole 66.7%. It was concludedthat biochemical characterization, Microbact test and antibiotic susceptibility test are essential forquick diagnosis and the selection of appropriate antibiotic agents for the treatment of fowl cholera